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395 €
This course will explain different molecular and cell biology techniques that are used most frequently in the field of assisted reproduction. Among them, we will consider techniques to study nucleic acids such as PCR, quantitative PCR, and expression arrays.
Regarding the study of proteins, we will include Western blot, ELISA, immunohistochemistry and global proteomic techniques such as DIGE or liquid chromatography coupled to mass spectrometry. In addition, we will also explain how to work in a cell culture laboratory, and we will detail several cell models that are usually handled in research such as immortalized cell lines or animal models such as rats or mice.
We will also study massive sequencing techniques, introduction to statistics and all aspects related to the elaboration of a research project.
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Objetivos
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Course intended for Biomedical Professionals, Biotechnologists, Andrologists and Embryologists.
Temario completo de este curso
1.Basic Research in Assisted Reproduction
2.Isolation techniques and study of nucleic acids
3.Isolation techniques and study of proteins
4.Morphological location. Immunohistochemistry. Confocal and electron microscopy
5.Cell culture. Culture of human endometrial epithelial and stromal cells
6.In vivo studies on animal models. Model for endometriosis studies
7.Omics as a non-invasive diagnostic tool
8.Massive sequencing applied to reproductive medicine
9.Mitochondrial DNA: the energy of the preimplantation embryo
10.Introduction to statistics I
11.Introduction to statistics II
12.Introduction to statistics III
13.Introduction to research and good clinical practices
14.Developing a research project
15.Functions and responsibilities of the participants in a clinical trial
16.Drug development process
17.Classification of research projects
18.Use of research results: publication of results
19.How to write a manuscript
20.Ethics within publications
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